Current Issue : October-December Volume : 2020 Issue Number : 4 Articles : 8 Articles
Entacapone is a particular, reversible catechol methyl transferase (COMT) inhibitor for the treatment of Parkinson's ailment entacapone is a selective and reversible inhibitor of catechol ortho methyltransferase (COMT), with essentially fringe actions. It is utilized in the treatment of Parkinson's malady as an extra to levodopa/carbidopa treatment. So it is extraordinary enthusiasm of study to outlines the investigative profile of ETP alone and in mix. The benefit relevance of ETP was found by HPLC technique and in this manner UV strategy. Less writing was accounted for by UPLC and LC MS-MS strategies. Such estimations consistently need exact pharmacological and physicochemical data; subsequently creators have introduced it in advance. The article further presents a concurrent and similar appraisal of the diagnostic investigations referencing pharmaceutical estimation of medication to aid current examination. The careful writing look through uncovered twenty five research papers altogether till date. Such information will aid the advancement of new conveyance frameworks in pharmaceutical research....
Reverse phase-high performance liquid chromatography (RP-HPLC) method have been developed and validated for the estimation of mifepristone and misoprostol. The developed method is rapid, accurate, precise, simple and specific. The separation was carried out using Hypersil C18 column (250 mm x 4.6 mm, 5µm i.d) as stationary phase in isocratic mode, with mobile phase containing Methanol: Buffer (0.01M Pentane sulphonic acid sodium salt pH 3.2 adjusted with OPA) (55:45v/v). The flow rate is 1 ml/min; detection was carried out at wavelength 220 nm. Chromatogram showed peak at a retention time of 2.750 min for mifepristone and 4.942 min for misoprostol. The method is validated for system suitability, linearity, precision, accuracy specificity, ruggedness, robustness, LOD and LOQ. Recovery of mifepristone and misoprostol is found to be in the range of 99.12 - 100.73% and 99.09 – 100.35%. LOD and LOQ were found to be 0.724 μg/ml and 2.196μg/ml for mifepristone respectively and 0.032μg/ml and 0.097 μg/ml for misoprostol respectively. Proposed method can be successfully applied for routine analysis of mifepristone and misoprostol in its pharmaceutical dosage form....
A new, simple, reliable and reproducible stability indicating RP-HPLC method simultaneous estimation of mifepristone and misoprostol in its oral solid dosage form. Effective chromatographic separations were achieved on a Hypersil C18, 250×4.6 mm, 5μ column with reverse phase elution. Methanol: Buffer (0.01M pentanesulfonic acid Sodium salt, pH 3.5 adjusted with OPA)(25:75v/v)as mobile phase and flow rate 1ml/min. Detection was carried out at wavelength 220nm. In Linearity was observed in the concentration rang 50-150µg/ml and 5-15µg/ml with correlation coefficient of mifepristone and misoprostol (R2) 0.998 and (R2) 0.997, respectively. The Mean recovery were found to be in the range 99.72-100.47% and 99.68 - 100.87%respectively. LOD and LOQ were found to be 1.297µg/ml and 3.931µg/ml for mifepristone and 0.0321 µg/ml and 0.0977µg/ml for misoprostol. The %RSD of repeatability precision, interday precision and intraday precision of mifepristone and misoprostol was found to be 0.9, 0.73 and 0.43 and 0.63, 0.74 and 1.03, respectively. It indicated that the method is precise. In forced degradation, the proposed method has been investigated with different stress conditions as hydrolytic, oxidative, thermal and Photo as recommended by ICH guidelines. An accurate and reliable RP-HPLC method for the analysis of mifepristone and misoprostol was developed and validated successfully....
A simple, accurate and validated liquid chromatographic method was developed for the estimation of rifampicin (RIF) and isoniazid (INH) in bulk and combined dosage forms. Drugs were injected in a chromatographic system on a reverse-phase C8 column using a mobile phase composition of Acetonitrile: Ammonium acetate buffer (50:50), isocratically and monitored at the analogous maximum of each compound. Peaks were recognized with a retention time as compared with standards and established with characteristic spectra using the PDA detector. The concentrations of the solutions were measured on a weight base to eliminate the use of an internal standard. The method was found linear (r 2 = 0.999 for RIF and 0.998 for INH), precise (RSD%: 1.56 for RIF and 0.82 for INH), accurate (overall average recovery yields: 100.1% for RIF and 100.59% for INH) and selective. Retention time (min) for RIF and INH was found 3.8 and 2.3 respectively. Because of simplicity and accuracy, the method is appropriate for regular quality control analysis of anti-tuberculosis bulk drug and dosage form. The proposed method of HPLC was developed and validated thoroughly for the quantitative analysis of RIF and INH in bulk and combined dosage form. The method was found to be simple, rapid, accurate, precise and economical and gives an acceptable recovery of the analytes, which can be directly and quickly applied for routine analysis in quality control of RIF and INH mixture in pharmaceutical bulk and formulations....
The objective of this study was to quantify the antibiotics (oxytetracycline and enrofloxacin) residue levels in raw and layers by RP-HPLC. This study was carried out on one hundred randomly collected raw and layers hen egg samples (20 of each) to evaluate the antibiotic (oxytetracycline and enrofloxacin) residues level qualitatively by high performance liquid chromatography (HPLC). The separation was carried out using mobile phase composed of 0.1%formic acid: Acetonitrile (50:50, v/v) using C18 (Hypersil ODS-BPS, 250 x 4.6mm; 5µ) at a flow rate of 1ml/min. The residues were quantified at detection wave length 350 nm. The obtained results revealed a detectable level of oxytetracycline and enrofloxacin residues which confirm widespread misuses of antibiotics in farms and lack of application of recommended withdrawal times. The boiling effect of broiler hen egg results shows the reduction in concentration of antibiotic residues. These findings recommended that restricted measures and harder regulations must be applied to prevent the misuse residues prior to marketing....
Hydroxylamine (HYX) is a vital class of reducing chemical compound, which is generally used as an industrial raw material in pharmaceutical reaction processes. The key concern is drug protection because if repeated exposure to GIT compounds that can damage DNA by alkylation leads to a mutation on genetic codes such impurities can react with mutation-causing DNA bases and have toxic effects such as genotoxicity and carcinogenicity. As per the Regulatory guidelines, Genotoxic impurities should be controlled to limits permitted by the Threshold of Toxicological Concern (TTC) the value was estimated to be 1.5 µg/day intake. Several methods have been reported to detect and quantify hydroxylamine most of which use a derivatization approach coupled with analytical techniques like HPLC, GC, UV-Vis Spectroscopy. In the HPLC method derivatizing agent was used to detect easily by UV detector and also the method is sensitive as its LOD was low. In the GC method with ECD most sensitive method was developed with LOD of 1.4ppb. In Spectrophotometric methods, a common problem that occurs is the loss of nitrite by using iodine solution to control this problem by using sodium arsenate and bromobenzene instead of iodine, but they are simple when compared with above two methods. To detect HYX in waste stream Ion chromatography method was developed which is more sensitive to detect trace quantities of hydroxylamine. LC-MS method is the best method, which is very sensitive to detect LOD of HYX up to 0.008ppm....
Bibhitakadi Ghrita (BG) is mentioned in Netrarogadhikara by Yogratnakara. Along with its modern management this drug might be useful in treating Presbyopia. Bibhitakadi Ghrita was processed as per the process of Snehapaka procedure described in classics. It contained Bibhitaki (Terminalia bellirica), Haritaki (Terminalia chebula), Amalaki (Embilica officinalis), Nimba (Azadirachta indica), Patola (Trichosanthes dioca), Vasa (Adhathoda vasica) and Go Ghrita. In the preparation of Bibhitakadi Ghrita, Water, Kalka Dravya of Bibhitaki, Haritaki, Amalaki, Nimba, Patola and Vasa were mixed in Go Ghrita and heated for three hours at 110°C every day for three days. On the third day Ghrita was filtered to obtain the finished product. Organoleptic features of Bibhitakadi Ghrita were observed like light to dark greenish brown, coloured greasy Ghrita with fragrant smell and bitter taste. In this manner, to understand the changes that occurred during the preparation, Bibhitakadi Ghrita was analyzed by using modern parameters such as acid value, saponification value and so on. After the analysis, evaluation of Bibhitakadi Ghrita showed that Refractive Index (At room Temperature) was 1.362, Loss on Drying was 0.78%, Ash value was 0.056% w/w, Acid value was 1.695 % w/w, Iodine value was 33% w/w, Saponification value was 240.0 % w/w and Total fat was 96.56%....
A novel, simple, precise and accurate area under curve spectroscopy method has been developed for estimation of capecitabine in bulk and pharmaceutical dosage form and AUC was measured at 301-311nm in methanol: acetonitrile: distilled water (70:20:10 v/v) and obeys Beer’s law in the concentration range of 2-10 µg/ml. The linearity study was carried and regression coefficient was found to be 0.9999 and it shows good linearity, precision in this concentration range. The regression equation was found to be Y = 0.0294X + 0.0007. The % recovery was found to be 99.85-100.39. The LOD and LOQ were found to be 0.055 and 0.165 µg/ml. The % relative standard deviation was found with in ± 2. The method has been validated according to ICH guidelines for linearity, precision, accuracy, robustness, ruggedness, LOD and LOQ. The developed validated method was successfully applied for reliable quantification of Capecitabine in bulk and pharmaceutical dosage form....
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